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| Topic: |
Technology
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| Number of pages / Number of words: |
7 / 1897 |
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In this process myloma-myloma and spleen cell-spleen cell fusions are eliminated leaving only spleen cell-myloma hybrids. The HAT growth medium contains aminopterin which is an inhibitor of the pathways that the homogenously fused cells use to make nucleotides and therefore DNA. So, the cells must use a bypass pathway that is defective in the myeloma cell line to which the normal antibody producing cells are fused...
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The HAT growth medium contains aminopterin which is an inhibitor of the pathways that the homogenously fused cells use to make nucleotides and therefore DNA. So, the cells must use a bypass pathway that is defective in the myeloma cell line to which the normal antibody producing cells are fused. Because neither the myeloma nor the antibody producing cell will grow on its own only the hybrids grow...
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