Buy custom Step-Wise Purification and Characterization of a Lactate Dehydrogenase Isozyme from unknown tissue sample of Sus domestica
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| Topic: |
Science
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| Number of pages / Number of words: |
6 / 1539 |
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The strategy employed used consecutively selective steps to end in an isolate of nearly pure LDH product.
Our approach was closely related to that used in the isolation of glutathione reductase from rainbow trout liver. Initially those authors prepared a homogenate and centrifuged it, followed by an ammonium sulfate fractionation, affinity chromatography and a gel filtration also known as size exclusion chromatography ...
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Our scheme was identical; however a step of gel electrophoresis was added at the end with standard preparations of LDH H4 and LDH M4 to identify the exact isozyme present.
Introduction: You will need to give some background information on LDH, enough to make the reader familiar with the enzyme you are working with...
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Step-Wise Purification and Characterization of a Lactate Dehydrogenase Isozyme from unknown tissue sample of Sus domestica
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