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| Topic: |
Science
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| Number of pages / Number of words: |
2 / 508 |
Essay's paper body
Therefore, brands of all different
lengths are produced. Once these reactions are completed, the DNA is again denatured in
preparation for electrophoresis. This is demonstrated below:
In electrophoresis, the denatured single stranded DNA molecules run on a
polyacrylamide gel. The gel is dried and exposed to X-ray film...
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Since the template strand
is not radiolabled, it does not generate a band on the x-ray film. The chain terminations
closest to the primer generate the smallest DNA molecules which migrate further down
the gel whereas the chain terminations further from the primer generate larger DNA
molecules which are slower on the gel and therefore closer to the top...
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