Buy custom Quenching of Fluorescence 
    
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            | Topic: | Science | 
        
            | Number of pages / Number of words: | 5 / 1387 | 
                
    
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As a result, the ratio of the intensity in the absence of quencher (I0) to that in the presence of a given concentration of quencher (I) is
In terms of ?, the equation becomes
Graphing I0/I with respect to the concentration of quencher will yield a line with slope kQ?i. The rate constant can then be determined if ?i is found by other means...
        
        
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The rate constant can then be determined if ?i is found by other means. The rate of the reaction is dependant on the rate at which the two substances diffuse together. This rate constant, the diffusion controlled bimolecular rate constant, k2, depend on the viscosity and temperature of the solvent. K2 is given by
where rA is equal to 6...
        
        
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