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Topic: |
Science
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Number of pages / Number of words: |
10 / 2672 |
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A equal volume of ammonia sulphate was solution was added slowly and the solution was stirred with a glass rod. This was allowed to stand 30 minutes and was occasional stirred.
The sample was then centrifuged down (3500rpm for 20minutes), the supernatant that contained the ovalbumin was placed into a new centrifuged tube...
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The volume of H2SO4 was recorded and the same volume of ammonia sulphate was added to solution which was stirred and left for 5 minutes to allow ovalbumin to precipitate out of solution.
The sample was once again centrifuged (3500rpm for 20minutes) to recover the precipitate of ovalbumin.
The sample was mixed with water and was placed into a small dialysis tubing to dialysed off the ammonium sulphate against running water over night...
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