Buy custom Proteomics: Investigation of sample loading techniques for Iso-Electric Focusing
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| Topic: |
Science
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| Number of pages / Number of words: |
16 / 4362 |
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Our objectives are to combine conditions to load complex protein samples onto IPG strips for iso-electric focusing gel (IEF), coupled with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and to evaluate the results by image analysis after silver staining.
Our hypothesis is that using both cup-loading and including the sample in the rehydration buffer will produce a gel with better resolution, in terms of more localized spots...
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After that, first dimension IEF and SDS-PAGE and silver staining were carried out.
Experiment 1 was found to produce clearer and more distinct spots. Although experiment 2 produced a gel with less distinct spots, it can be further improved on by modifying the focusing time for IEF.
Background to first dimension iso-electric focusing (IEF) 1
In IEF, proteins are separated on the basis of isoelectric points (pI)...
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