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Topic: |
Science
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Number of pages / Number of words: |
3 / 723 |
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The results that should have been observed by the group, are very small line at A (the standard DNA Fragments), At B (Control Sample after 0 cycles) there should have been an extremely
small line. The wells for C (sample after 10 Cycles), D (sample after 30 cycles) and E (sample after 50 cycles) should progressavly get large from one to another...
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F should be the longest and most easily seen line, because this process was run overnight, which allowed more time for more DNA to be prepared.
Questions:
1) Upon reducing with B-mercaptoethanol, total amino analysis of E.coli DNA polymerase 1 indicates the presences of three cysteine residues. How do you rationalize this data based on the information provided?
Due to the fact that cysteine is hydrophilic, this leads the results to be that
B-mercaptoethanol is also classified as hydrophilic...
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