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This ratio is known as transmittance of the solution, T:
o
? To ascertain the amount of light absorbed by the solution, which is used to determine the concentration of the solution, we can use the following equation that relates Absorption, A, and transmittance, T:
o
? Note that as A decreases, T increases meaning that the more there is transmitted light, there is less absorption, and vice versa...
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Each species is unique in this.
o As the path length of the light is increased (determined by the width of the cuvette), the more light is absorbed.
o Beer-Lambert's Law relates all molar absorptivity coefficient, concentration, and path length of light:
?
? Where A is absorption of the solution, ? is the molar absorptivity coefficient of the absorbing species, b is the path length, and c is the concentration of the solution...
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