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Science
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| Number of pages / Number of words: |
8 / 2165 |
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The reaction mixture is taken through the following replication cycles (also, see Figure 2):
? 1 to several minutes at 94-96°C, during which the DNA is denatured into single strands,
? 1 to several minutes at 50-65°C, during which the primers form base pairs, or anneal, to their complementary sequences on either side of the target DNA sequence, and
? 1 to several minutes at 72°C, during which the polymerase binds and extends a complementary DNA strand from each primer...
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The reaction mixture is taken through the following replication cycles (also, see Figure 2):
? 1 to several minutes at 94-96°C, during which the DNA is denatured into single strands,
? 1 to several minutes at 50-65°C, during which the primers form base pairs, or anneal, to their complementary sequences on either side of the target DNA sequence, and
? 1 to several minutes at 72°C, during which the polymerase binds and extends a complementary DNA strand from each primer...
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