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| Topic: |
Science
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| Number of pages / Number of words: |
4 / 998 |
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Afterwards it was observed for color change. A positive result would have a color change of pink=rapid urea hydrolysis, strong urease production and orange or yellow=no urea hydrolysis, organism does not produce urease or cannot live in broth.
The SIM Tube was incubated for 24-48 hrs at 37?C. Afterwards it was observed for spreading from the stab line, and black precipitate in the medium...
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Afterwards it was observed for spreading from the stab line, and black precipitate in the medium. Next Kovacs Reagent was added to tube and after a minute observed for the formation of red color in the reagent layer. The results for Sulfur present would be positive if black in the medium=sulfur reduction (H2S Production), negative if no black in medium=Sulfur is not reduced...
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