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| Topic: |
Science
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| Number of pages / Number of words: |
18 / 4906 |
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The water in the paper fibres acts as the stationary phase as the solvent flows by capillary action up the paper in ascending chromatography. One solvent, usually water, is adsorbed on the cellulose in the paper; adsorption is the concentration of one substance at the surface of another. The other solvent, such as a mixture of 1-butanol and ethanoic acid (usually called diluting agent), travels up the paper...
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The other solvent, such as a mixture of 1-butanol and ethanoic acid (usually called diluting agent), travels up the paper. Each amino acid has a particular solubility in the diluting and stationary solvent. Amino acids with higher solubility in diluting solvent will travel higher up in ascending chromatography...
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