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| Topic: |
Science
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| Number of pages / Number of words: |
6 / 1623 |
Essay's paper body
let the plates rest for several minutes
D. remove the glass beads
15. Transfer 100uL of cell suspension from the +plasmid tube to each appropriate plate
16. Immediately suspend the cells
17. Wrap the plates together with tape and place the plates upside down in the incubator.
Purification of GFP by HIC Steps
1...
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Shake the culture tube to suspend the E.coli cells.
2. Use a micropipette to transfer 1mL of the overnight E.coli/GFP culture into a 1.5mL
tube.
3. Cap the tube and place it in a balanced configuration in the micro centrifuge rotor. Spin for one minute to pellet the cells.
4. Carefully pour off the supernatant...
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