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| Topic: |
Science
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| Number of pages / Number of words: |
5 / 1185 |
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In this process small DNA fragments of a known length are made, using a polymerase chain reaction. These frag-ments contain biotin molecules, usually vitamin H, covalently attached to each end. Then the DNA is labeled with streptavidin. This tetrameric complex was expected to bind up to four DNA molecules via their attached biotin molecules...
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The DNA is then imaged with atomic force microscopy (AFM). Images revealed the protein at the end of the DNA strands as well as the presence of dimers, trimers, and tetramers of DNA bound to a single protein. Imaging time was about 1 min.
With these results, we believe we have shown that AFM does have sufficient resolution to map DNA...
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