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Topic: |
Science
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Number of pages / Number of words: |
12 / 3189 |
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The cohesive ends of the DNA and plasmid line up and the enzyme DNA ligase joins the fragments by forming phosphodiester bonds11.
http://agbiosafety.unl.edu/education/clone.htm
The newly formed recombinant DNA is then transferred back into Agrobacterium tumefaciens using electroporation or heat shock11...
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Both of these techniques disrupt the bacteriaâs cell wall and create small holes11. The recombinant DNA is small enough to pass through these holes into the cell11.
The bacteria are then cultured in nutrient dishes and grow into colonies11. The bacteria are treated with an antibiotic, such as Kanamycin, for which the genetically modified plasmid carries resistance to2...
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