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Science
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| Number of pages / Number of words: |
13 / 3527 |
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Cheesecloth filtration, repetitive centrifugation and discarding of sediments are performed afterwards to remove unwanted components such as the added sand or the ruptured cell walls. The precipitation of invertase is made possible by immersion of the final supernatant in an ice bath and pouring of 95% ethanol...
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The precipitation of invertase is made possible by immersion of the final supernatant in an ice bath and pouring of 95% ethanol. The method is based fundamentally on the principle regarding dielectric constants. Upon introduction of the alcohol, “the dielectric constant is lowered and the attractions between the ionizable groups of the enzyme are increased...
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