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| Topic: |
Science
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| Number of pages / Number of words: |
7 / 1747 |
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To do this we will use para-nitrophenyl phosphate (PNPP) as the substrate. We are using this substrate because one of the products (para-nitrophenol) has a very high molar extinction coefficient; a405 = 18,800 M -1 cm -1. This allows us to measure very small quantities of the product easily. However, only the para-nitrophenolate ion absorbs...
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This allows us to measure very small quantities of the product easily. However, only the para-nitrophenolate ion absorbs.
O2NOPO32-O2NOH+HOPO32-
Previously we used Acid Phosphatase for this experiment. As the name suggests this enzyme is active at acidic pH values. Because para-
nitrophenol has a pKa of about 7...
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