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Four 50 mL beakers were filled with the following solutions, with a corresponding pipette for each one: pH 5 buffer, hydrogen peroxide, the extract, and guaiacol. Seven test tubes were prepared as follows:

Table 1: Mixing table for standardization

Test tube # pH 5 buffer (mL) H2O2 (mL) Extract (mL) Guaiacol (mL) Total volume (mL)

1 5...

Seven test tubes were prepared as follows:

Table 1: Mixing table for standardization

Test tube # pH 5 buffer (mL) H2O2 (mL) Extract (mL) Guaiacol (mL) Total volume (mL)

1 5.0 0 2.0 1.0 8

2 0 2.0 0 1.0 3

3 4.5 0 0.5 0 5

4 0 2.0 0 1.0 3

5 4.0 0 1.0 0 5

6 0 2.0 0 1.0 3

7 3.0 0 2.0 0 5

The contents of test tube 1 are used to blank the spectrophotometer to zero absorbance at 500 nm...

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