Buy custom Determining the source of excess phosphatase in hypothetical patient
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| Topic: |
Science
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| Number of pages / Number of words: |
5 / 1250 |
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Recent studies suggest hepatic metabolism regulation by the inhibition of NF-?B activity can improve glucose tolerance and may provide a new treatment option for type 2 Diabetes (Tamura et al 2007) and EDTA can reactivate aminopeptidase N activity suppressed by metal ion chelators (Vanderheyden et al 2006)...
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In our experiment, the source of excess phosphatase was determined for a patient with unknown symptoms based on the biochemical properties of the isozyme using different assays based on pH and reaction to potential inhibitors. Enzymatic activity was determined after incubation with pNPP substrate by the addition NaOH, which caused the reaction product nitrophenol to turn yellow...
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