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Science
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| Number of pages / Number of words: |
22 / 6024 |
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then carry out complex DNA analysis . plot against time, when ss, absorption of light increases (almost two fold) . Melting temperature (important) or TM is a function of the G & C content.
Back to the question – if an org just has unique sequence (NO repeats of several times in the genome), cut into pieces (~1000bp), denature, then the renaturation will take a long time for those 2 complimentary strands to come together (Cot value will be large)...
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However if sequences are repeated several times, then the renaturation will be rapid (the cot value will be small (assumption) – DIAGRAM 1 tells you molecule is 30X more complex
DIA 2 – 1. reassoc rapidly (lots of repeating sequences), 2. reassociates moderately, 3. reassociates slowly. Notice the length or the steepness ( can tell you the relative allocation of the genome to that particular complexity)
DIA 3 – Cot = DNA [ ] (m) x time (s)
Cot ? - calue of ? reassociation
The diagram shows that 60% is highly repeated, 25% moderately repeated, 15% unique
Ecoli seq are unique where as mouse DNA is highly repetitive (106 copies, 300 bp average, 15%) , 25% mod repetitive (103 tp 104 copies) several seeuees, 60% are unique, single or few copies, genes coding for mRNAs (how do we know this?? ? use mRNA – DNA hybridization – so when you go to the cyto you’ll find mRNA – convert them to ***cDNAs or the mRNA tagged...
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