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| Topic: |
Science
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| Number of pages / Number of words: |
4 / 845 |
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For a and a sites mutations were made to attach there. This was done so that both agonist binding sites were disturbed. With there unnatural amino acids mutant proteins were expressed on the Xenopus oocytes. Once expressed their whole cell currents were taken. The current is used to open the receptor...
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It was established that large observed large currents were due to the nicotinic acetylcholine receptor rather than the Xenopus oocyte. Only three of the evaluated sites produced significant enough current, indicating an active receptor, to be considered for further analysis. These sites were then treated with the natural amino acid of the nicotinic acetylcholine receptor...
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