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While attached to the preservation apparatus, samples of the media output of the heart were taken every 2 hours and were assayed for their content. If the compound in the media showed up in large amounts in the assay, it could be concluded that the compound was not metabolized by the heart. If little or none of the compound placed in the media appeared in the assay, it could be concluded that compound was used up by the heart metabolism...
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If little or none of the compound placed in the media appeared in the assay, it could be concluded that compound was used up by the heart metabolism.
The hearts that were given pyruvate in their media completely consumed the available substrate and were able to function at a nearly normal capacity once they were transplanted...
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