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Smear it onto the slide and air dry.
8. Flame the loop, cool and take one sample of colony B in that same section. Smear it onto the slide and air dry.
9. Repeat steps numbers 6-8 till all 12 slides are completed.
10. Start with the 1st air dried slide and heat fix, stopping at the 12th air dried slide...
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Use the clothes pin to grasp the slide, pass thru the flame 3-4 times quickly.
12. Heat fixes all 12 slides.
13. perform a gram stain on all 12 slides
14. Cover the smear with crystal violet and leave on for 30-45 seconds.
15. Rinse with distilled water.
16. cover the smear with iodine(mordant) for 10 seconds
17...
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