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You can select the nature and strength of the interactions by your choice of phases. The components move with different speeds through the column, depending on their affinity for the different phases, and so they elute from the column at different times: the retention times. By changing the composition of the mobile phase during the elution, its possible to analyze a wider variety of compounds in a given time than would be possible under constant conditions...
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By changing the composition of the mobile phase during the elution, its possible to analyze a wider variety of compounds in a given time than would be possible under constant conditions.
Chromatography is used in chemistry and biochemistry research analyzing complex mixtures, purifying chemical compounds, developing processes for synthesizing chemical compounds, isolating natural products, or predicting physical properties...
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